Centromeric Chromatin and Epigenetic Effects in Kinetochore Assembly
نویسندگان
چکیده
proteins, such as transcription factors, bind selectively to DNA via short but highly conserved binding sites, a second class of factors such as yeast centromere binding factor III (CBF3; Espelin et al., 1997) and the origin recognition complex (ORC) bind to large, poorly conGarth R. Wiens and Peter K. Sorger Department of Biology Massachusetts Institute of Technology Cambridge, Massachusetts 02139
منابع مشابه
Octameric CENP-A Nucleosomes Are Present at Human Centromeres throughout the Cell Cycle
The presence of a single centromere on each chromosome that signals formation of a mitotic kinetochore is central to accurate chromosome segregation. The histone H3 variant centromere protein-A (CENP-A) is critical for centromere identity and function; CENP-A chromatin acts as an epigenetic mark to direct both centromere and kinetochore assembly. Interpreting the centromere epigenetic mark ensu...
متن کاملHistone H4 Lys 20 Monomethylation of the CENP-A Nucleosome Is Essential for Kinetochore Assembly
In vertebrate cells, centromeres are specified epigenetically through the deposition of the centromere-specific histone CENP-A. Following CENP-A deposition, additional proteins are assembled on centromeric chromatin. However, it remains unknown whether additional epigenetic features of centromeric chromatin are required for kinetochore assembly. Here, we used ChIP-seq analysis to examine centro...
متن کاملChromatin containing CENP-A and α-satellite DNA is a major component of the inner kinetochore plate
The pathway of molecular interactions leading to kinetochore assembly on mammalian chromosomes is unknown. Kinetochores could be specified by structural features of centromeric satellite DNA [1-3] or by specific DNA sequences, analogous to budding yeast centromeres, interspersed in centromeric satellite DNA arrays [4,5]. Alternatively, kinetochores could be epigenetic structures that replicate ...
متن کاملThe role of heterochromatin in centromere function.
Chromatin at centromeres is distinct from the chromatin in which the remainder of the genome is assembled. Two features consistently distinguish centromeres: the presence of the histone H3 variant CENP-A and, in most organisms, the presence of heterochromatin. In fission yeast, domains of silent "heterochromatin" flank the CENP-A chromatin domain that forms a platform upon which the kinetochore...
متن کاملHeterochromatin and RNAi are required to establish CENP-A chromatin at centromeres.
Heterochromatin is defined by distinct posttranslational modifications on histones, such as methylation of histone H3 at lysine 9 (H3K9), which allows heterochromatin protein 1 (HP1)-related chromodomain proteins to bind. Heterochromatin is frequently found near CENP-A chromatin, which is the key determinant of kinetochore assembly. We have discovered that the RNA interference (RNAi)-directed h...
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عنوان ژورنال:
- Cell
دوره 93 شماره
صفحات -
تاریخ انتشار 1998